This book summarizes the principles, protocols, and applications of enzyme- based biosensors in healthcare. After providing a brief history and discussing the development of various biosensors used for medical applications, it explores the principles of enzyme-based biosensors and reviews a number of enzyme immobilization techniques. It then addresses the potential of enzyme- based biosensors in the diagnosis of infectious and non-infectious diseases; illustrates the potential of enzyme-based biosensors for non-invasive monitoring of biochemical biomarkers; and assesses the application of paper- based and microfluidic-based nanobiosensors in medical diagnostics. A one-stop resource on recent advances in enzyme-based biosensors in healthcare, this book will appeal to students, researchers and