After introduction of strawberry cultivation in Bangladesh and development of new varieties from a Japanese genotype at the Department of Botany, Rajshahi University, Bangladesh, strawberry cultivation and its popularity as a cash crop have been rapidly increased. For further improvement, it is important to develop more adaptable varieties for large-scale production of strawberry in Bangladesh. Leaf explants were cultured on MS medium with 2,4-D, or NAA alone or with BA for callus induction. Calli were cultured for shoot regeneration in MS medium with BA alone or with BA + NAA and BA + NAA + KIN. After successful regeneration, shoots were rooted in MS0 and the