this Second Edition. Readers benefit from the author's twenty-five years of experience in HPLC, first as a user, then as a field sales and application support representative, and finally as a teacher and consultant. The book's wealth of tips and troubleshooting advice originate from the author's own laboratory and field work. HPLC's structure is carefully designed to give readers a solid foundationin the basics and then methodically build and refine their skills: * Part I, HPLC Primer, presents the essentials needed to get an HPLC system up and running * Part II, HPLC Optimization, explains how to make the best use of the common columns and troubleshoot problems * Part III, HPLC Utilization, focuses on real-world applications, including interfacing HPLC systems with computers and robotic workstations A CD-ROM with the author's PowerPoint(r) presentation, Practical Course in HPLC, is included with the book. Plus, new appendices offer answers to frequently asked questions, tables of solvents and volatile buffers, an updated glossary of terms, and laboratory experiments. In short, everything that you need to know to confidently separate, identify, purify, and quantify compounds is here.